Widespread rearrangement of 3D chromatin organization underlies polycomb-mediated stress-induced silencing.

ABSTRACT: Chromosomes of metazoan organisms are partitioned in the interphase nucleus into discrete topologically associating domains (TADs). Borders between TADs are formed in regions containing active genes and clusters of architectural protein binding sites. The transcription of most genes is repressed after temperature stress in Drosophila. Here we show that temperature stress induces relocalization of architectural proteins from TAD borders to inside TADs, and this is accompanied by a dramatic rearrangement in the 3D organization of the nucleus. TAD border strength declines, allowing for an increase in long-distance inter-TAD interactions. Similar but quantitatively weaker effects are observed upon inhibition of transcription or depletion of individual architectural proteins. Heat shock-induced

SUBMITTER: Li L 

PROVIDER: S-EPMC4402144 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature