Characterization of a novel antisense RNA in the major pilin locus of Neisseria meningitidis influencing antigenic variation.

ABSTRACT:

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Expression of type four pili (Tfp) is essential for virulence in Neisseria meningitidis. Pili mediate adhesion, bacterial aggregation, and DNA uptake. In N. meningitidis, the major pilin subunit is encoded by the pilE gene. In some strains, PilE is subject to phase and antigenic variation, which can alter Tfp properties and together offer a possible mechanism of immune escape. Pilin expression and antigenic variation can be modulated in response to environmental cues; however, the precise mechanisms of such regulation remain unclear. We identified a promoter in the pilE locus, 3' of the pilE coding sequence, on the antisense (AS) strand which is conserved in meningococci. We show that this promoter directs transcription of an AS RNA that is expressed during specific grow

SUBMITTER: Tan FY 

PROVIDER: S-EPMC4402397 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature