Increased functional protein expression using nucleotide sequence features enriched in highly expressed genes in zebrafish.

ABSTRACT: Many genetic manipulations are limited by difficulty in obtaining adequate levels of protein expression. Bioinformatic and experimental studies have identified nucleotide sequence features that may increase expression, however it is difficult to assess the relative influence of these features. Zebrafish embryos are rapidly injected with calibrated doses of mRNA, enabling the effects of multiple sequence changes to be compared in vivo. Using RNAseq and microarray data, we identified a set of genes that are highly expressed in zebrafish embryos and systematically analyzed for enrichment of sequence features correlated with levels of protein expression. We then tested enriched features by embryo microinjection and functional tests of multiple protein reporters. Codon selection, releasing fact

SUBMITTER: Horstick EJ 

PROVIDER: S-EPMC4402511 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature