Metabolite localization in living drosophila using High Resolution Magic Angle Spinning NMR.

ABSTRACT: We have developed new methods enabling in vivo localization and identification of metabolites through their (1)H NMR signatures, in a drosophila. Metabolic profiles in localized regions were obtained using HR-MAS Slice Localized Spectroscopy and Chemical Shift Imaging at high magnetic fields. These methods enabled measurement of metabolite contents in anatomic regions of the fly, demonstrated by a decrease in β-alanine signals in the thorax of flies showing muscle degeneration.

SUBMITTER: Sarou-Kanian V 

PROVIDER: S-EPMC4402646 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature