Expression of cancer/testis antigens in prostate cancer is associated with disease progression.

ABSTRACT:

Background

The cancer/testis antigens (CTAs) are a unique group of proteins normally expressed in germ cells but aberrantly expressed in several types of cancers including prostate cancer (PCa). However, their role in PCa has not been fully explored.

Methods

CTA expression profiling in PCa samples and cell lines was done utilizing a custom microarray that contained probes for two-thirds of all CTAs. The data were validated by quantitative PCR (Q-PCR). Functional studies were carried out by silencing gene expression with siRNA. DNA methylation was determined by methylation-specific PCR.

Results

A majority of CTAs expressed in PCa are located on the X chromosome (CT-X antigens). Several CT-X antigens from the MAGEA/CSAG subfamilies are coordinately upregulated in castra

SUBMITTER: Suyama T 

PROVIDER: S-EPMC4403006 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature