Characterization of hepatitis C virus interaction with heparan sulfate proteoglycans.

ABSTRACT:

Unlabelled

Hepatitis C virus (HCV) entry involves binding to cell surface heparan sulfate (HS) structures. However, due to the lipoprotein-like structure of HCV, the exact contribution of virion components to this interaction remains controversial. Here, we investigated the relative contribution of HCV envelope proteins and apolipoprotein E in the HS-binding step. Deletion of hypervariable region 1, a region previously proposed to be involved in HS binding, did not alter HCV virion binding to HS, indicating that this region is not involved in this interaction in the context of a viral infection. Patient sera and monoclonal antibodies recognizing different regions of HCV envelope glycoproteins were also used in a pulldown assay with beads coated with heparin, a close HS structural h

SUBMITTER: Xu Y 

PROVIDER: S-EPMC4403428 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature