Activation of NF-κB by human papillomavirus 16 E1 limits E1-dependent viral replication through degradation of E1.

ABSTRACT:

Unlabelled

NF-κB is a family of transcription factors that regulate gene expression involved in many processes, such as the inflammatory response and cancer progression. Little is known about associations of NF-κB with the human papillomavirus (HPV) life cycle. We have developed a tissue culture system to conditionally induce E1-dependent replication of the human papillomavirus 16 (HPV16) genome in human cervical keratinocytes and found that expression of HPV16 E1, a viral helicase, results in reduction of IκBα and subsequent activation of NF-κB in a manner dependent on helicase activity. Exogenous expression of a degradation-resistant mutant of IκBα, which inhibits the activation of NF-κB, enhanced E1-dependent replication of the viral genome. Wortmannin, a broad inhibitor of phos

SUBMITTER: Nakahara T 

PROVIDER: S-EPMC4403482 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature