Morphological and molecular discrimination of fasciola species isolated from domestic ruminants of urmia city, iran.

ABSTRACT:

Background

The trematodes of the genus Fasciola (the liver flukes) are among the well-known instances of food-borne parasites worldwide. Differentiation of Fasciola species is important because of their different transmission and epidemiological characteristics. The current study was undertaken to discriminate Fasciola species in the domestic ruminants of Urmia city, Iran.

Methods

Adult flukes were isolated from the naturally infected livers of the slaughtered water buffaloes and sheep. The flukes were initially identified based on morphological and morphometric parameters. A 618-bp-long fragment of the 28SrRNA gene of Fasciola was amplified by polymerase chain reaction (PCR). The amplified fragment was digested by DraII or AvaII enzymes for a restriction fragment length pol

SUBMITTER: Yakhchali M 

PROVIDER: S-EPMC4403539 | biostudies-literature | 2015 Jan-Mar

REPOSITORIES: biostudies-literature