Molecular characterization of eimeria species naturally infecting egyptian baldi chickens.

ABSTRACT:

Background

Coccidiosis is a serious protozoal disease of poultry. The identification of Eimeria species has important implications for diagnosis and control as well as for epidemiology. The molecular characterization of Eimeria species infecting Egyptian baladi chickens was investigated.

Methods

Eimeria species oocysts were harvested from intestines of naturally infected Egyptian baldi chickens. The morphometry characterization of oocysts along with COCCIMORPH software was done. The DNA was extracted initially by freezing and thawing then the prepared samples was subjected to commercial DNA kits. The DNA products were analyzed through conventional polymerase chain reaction by using amplified region (SCAR) marker.

Results

The PCR results confirmed the presence of 7 Eim

SUBMITTER: Gadelhaq SM 

PROVIDER: S-EPMC4403544 | biostudies-literature | 2015 Jan-Mar

REPOSITORIES: biostudies-literature