De novo assembly of the chimpanzee transcriptome from NextGen mRNA sequences.

ABSTRACT:

Background

Common chimpanzees (Pan troglodytes) and bonobos (Pan paniscus) are the species most closely related to humans. For this reason, it is especially important to have complete and accurate chimpanzee nucleotide and protein sequences to understand how humans evolved their unique capabilities. We provide transcriptome data from four untransformed cell types derived from the reference Pan troglodytes, "Clint", to better annotate the chimpanzee genome and provide empirical validation for proposed gene models of this important species.

Findings

RNA was extracted from primary cells cultured from four tissues: skin, adipose stroma, vascular smooth muscle and skeletal muscle. These four RNA samples were sequenced on the Illumina HiSeq 2000 platform. Sequences were deposited

SUBMITTER: Maudhoo MD 

PROVIDER: S-EPMC4403674 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature