Transcriptomic insights on the ABC transporter gene family in the salmon louse Caligus rogercresseyi.

ABSTRACT:

Background

ATP-binding cassette (ABC) protein family encode for membrane proteins involved in the transport of various biomolecules through the cellular membrane. These proteins have been identified in all taxa and present important physiological functions, including the process of insecticide detoxification in arthropods. For that reason the ectoparasite Caligus rogercresseyi represents a model species for understanding the molecular underpinnings involved in insecticide drug resistance.

Methods

llumina sequencing was performed using sea lice exposed to 2 and 3 ppb of deltamethrin and azamethiphos. Contigs obtained from de novo assembly were annotated by Blastx. RNA-Seq analysis was performed and validated by qPCR analysis.

Results

From the transcriptome database of

SUBMITTER: Valenzuela-Munoz V 

PROVIDER: S-EPMC4403882 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature