Leaps and lulls in the developmental transcriptome of Dictyostelium discoideum.

ABSTRACT:

Background

Development of the soil amoeba Dictyostelium discoideum is triggered by starvation. When placed on a solid substrate, the starving solitary amoebae cease growth, communicate via extracellular cAMP, aggregate by tens of thousands and develop into multicellular organisms. Early phases of the developmental program are often studied in cells starved in suspension while cAMP is provided exogenously. Previous studies revealed massive shifts in the transcriptome under both developmental conditions and a close relationship between gene expression and morphogenesis, but were limited by the sampling frequency and the resolution of the methods.

Results

Here, we combine the superior depth and specificity of RNA-seq-based analysis of mRNA abundance with high frequency sampling

SUBMITTER: Rosengarten RD 

PROVIDER: S-EPMC4403905 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature