The spliceosome factor SART1 exerts its anti-HCV action through mRNA splicing.

ABSTRACT:

Background &/aims

The broadly used antiviral cytokine interferon-α (IFNα)'s mechanisms of action against HCV infection are not well understood. We previously identified SART1, a host protein involved in RNA splicing and pre-mRNA processing, as a regulator of IFN's antiviral effects. We hypothesized that SART1 regulates antiviral IFN effector genes (IEGs) through mRNA processing and splicing.

Methods

We performed siRNA knockdown in HuH7.5.1 cells and mRNA-sequencing with or without IFN treatment. Selected gene mRNA variants and their proteins, together with HCV replication, were monitored by qRT-PCR and Western blot in HCV OR6 replicon cells and the JFH1 HCV infectious model.

Results

We identified 419 genes with a greater than 2-fold expression difference between Neg s

SUBMITTER: Lin W 

PROVIDER: S-EPMC4404186 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature