Site-specific programming of the host epithelial transcriptome by the gut microbiota.

ABSTRACT:

Background

The intestinal epithelium separates us from the microbiota but also interacts with it and thus affects host immune status and physiology. Previous studies investigated microbiota-induced responses in the gut using intact tissues or unfractionated epithelial cells, thereby limiting conclusions about regional differences in the epithelium. Here, we sought to investigate microbiota-induced transcriptional responses in specific fractions of intestinal epithelial cells. To this end, we used microarray analysis of laser capture microdissection (LCM)-harvested ileal and colonic tip and crypt epithelial fractions from germ-free and conventionally raised mice and from mice during the time course of colonization.

Results

We found that about 10% of the host's transcriptome w

SUBMITTER: Sommer F 

PROVIDER: S-EPMC4404278 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature