The Role of Alveolar Epithelial Type II-Like Cells in Uptake of Structurally Different Antigens and in Polarisation of Local Immune Responses.

ABSTRACT:

Background

Our previous studies on intranasal tolerance induction demonstrated reduction of allergic responses with different allergen constructs. The underlying mechanisms varied depending on their conformation or size.

Objective

The aim of the present study was to compare the uptake of two structurally different allergen molecules within the respiratory tract following intranasal application.

Methods

The three-dimensional Bet v 1 (Bv1-Protein) and the T cell epitope peptide of Bet v 1 (Bv1-Peptide) were labelled with 5,6-Carboxyfluorescein (FAM) and their uptake was investigated in lung cells and cells of the nasal associated lymphoid tissue from naive and sensitised BALB/c mice. Phenotypic characterisation of FAM+ lung cells after antigen incubation in vitro and af

SUBMITTER: Akgun J 

PROVIDER: S-EPMC4404363 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature