Global scale transcriptome analysis of Arabidopsis embryogenesis in vitro.

ABSTRACT:

Background

Somatic embryogenesis (SE) in plants is a process by which embryos are generated directly from somatic cells, rather than from the fused products of male and female gametes. Despite the detailed expression analysis of several somatic-to-embryonic marker genes, a comprehensive understanding of SE at a molecular level is still lacking. The present study was designed to generate high resolution transcriptome datasets for early SE providing the way for future research to understand the underlying molecular mechanisms that regulate this process. We sequenced Arabidopsis thaliana somatic embryos collected from three distinct developmental time-points (5, 10 and 15 d after in vitro culture) using the Illumina HiSeq 2000 platform.

Results

This study yielded a total of 426

SUBMITTER: Wickramasuriya AM 

PROVIDER: S-EPMC4404573 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature