Knockin' on pollen's door: live cell imaging of early polarization events in germinating Arabidopsis pollen.

ABSTRACT: Pollen tubes are an excellent system for studying the cellular dynamics and complex signaling pathways that coordinate polarized tip growth. Although several signaling mechanisms acting in the tip-growing pollen tube have been described, our knowledge on the subcellular and molecular events during pollen germination and growth site selection at the pollen plasma membrane is rather scarce. To simultaneously track germinating pollen from up to 12 genetically different plants we developed an inexpensive and easy mounting technique, suitable for every standard microscope setup. We performed high magnification live-cell imaging during Arabidopsis pollen activation, germination, and the establishment of pollen tube tip growth by using fluorescent marker lines labeling either the pollen cytoplasm

SUBMITTER: Vogler F 

PROVIDER: S-EPMC4404733 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature