CD8⁺/FOXP3⁺-ratio in osteosarcoma microenvironment separates survivors from non-survivors: a multicenter validated retrospective study.

ABSTRACT: Osteosarcoma is the most common primary bone tumor characterized by juvenile onset, tumor heterogeneity, and early pulmonary metastasis. Therapeutic improvement stagnates since more than two decades. Unlike major malignancies, biomarkers as prognostic factors at time of diagnosis are missing. Disease rareness hampers study recruitment of patient numbers sufficient to outweigh tumor heterogeneity. Here, we analyzed in a multicenter cohort the osteosarcoma microenvironment to reduce effects of tumor cell heterogeneity. We hypothesized that quantitative ratios of intratumoral CD8⁺T-cells to FOXP3⁺T-cells (CD8⁺/FOXP3⁺-ratios) provide strong prognostic information when analyzed by whole-slide imaging in diagnostic biopsies. We followed recommendations

SUBMITTER: Fritzsching B 

PROVIDER: S-EPMC4404826 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature