Inducible and combinatorial gene manipulation in mouse brain.

ABSTRACT: We have deployed recombinant adeno-associated viruses equipped with tetracycline-controlled genetic switches to manipulate gene expression in mouse brain. Here, we show a combinatorial genetic approach for inducible, cell type-specific gene expression and Cre/loxP mediated gene recombination in different brain regions. Our chemical-genetic approach will help to investigate 'when', 'where', and 'how' gene(s) control neuronal circuit dynamics, and organize, for example, sensory signal processing, learning and memory, and behavior.

SUBMITTER: Dogbevia GK 

PROVIDER: S-EPMC4404871 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature