Characterization of the Vibrio cholerae extracellular matrix: a top-down solid-state NMR approach.

ABSTRACT: Bacterial biofilms are communities of bacterial cells surrounded by a self-secreted extracellular matrix. Biofilm formation by Vibrio cholerae, the human pathogen responsible for cholera, contributes to its environmental survival and infectivity. Important genetic and molecular requirements have been identified for V. cholerae biofilm formation, yet a compositional accounting of these parts in the intact biofilm or extracellular matrix has not been described. As insoluble and non-crystalline assemblies, determinations of biofilm composition pose a challenge to conventional biochemical and biophysical analyses. The V. cholerae extracellular matrix composition is particularly complex with several proteins, complex polysaccharides, and other biomolecules having been identified as matrix parts

SUBMITTER: Reichhardt C 

PROVIDER: S-EPMC4406247 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature