Mutational analysis of circulating tumor cells from colorectal cancer patients and correlation with primary tumor tissue.

ABSTRACT: Circulating tumor cells (CTCs) provide a non-invasive accessible source of tumor material from patients with cancer. The cellular heterogeneity within CTC populations is of great clinical importance regarding the increasing number of adjuvant treatment options for patients with metastatic carcinomas, in order to eliminate residual disease. Moreover, the molecular profiling of these rare cells might lead to insight on disease progression and therapeutic strategies than simple CTCs counting. In the present study we investigated the feasibility to detect KRAS, BRAF, CD133 and Plastin3 (PLS3) mutations in an enriched CTCs cell suspension from patients with colorectal cancer, with the hypothesis that these genes` mutations are of great importance regarding the generation of CTCs subpopulations.

SUBMITTER: Lyberopoulou A 

PROVIDER: S-EPMC4406761 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature