Cardiac transcriptome and dilated cardiomyopathy genes in zebrafish.

ABSTRACT:

Background

Genetic studies of cardiomyopathy and heart failure have limited throughput in mammalian models. Adult zebrafish have been recently pursued as a vertebrate model with higher throughput, but genetic conservation must be tested.

Methods and results

We conducted transcriptome analysis of zebrafish heart and searched for fish homologues of 51 known human dilated cardiomyopathy-associated genes. We also identified genes with high cardiac expression and genes with differential expression between embryonic and adult stages. Among tested genes, 30 had a single zebrafish orthologue, 14 had 2 homologues, and 5 had ≥3 homologues. By analyzing the expression data on the basis of cardiac abundance and enrichment hypotheses, we identified a single zebrafish gene for 14 of 19 mu

SUBMITTER: Shih YH 

PROVIDER: S-EPMC4406804 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature