Blocking IKKα expression inhibits prostate cancer invasiveness.

ABSTRACT:

Purpose

IKKα has been recently identified as a key mediator of the inflammation and metastasis in prostate cancer. In the present study, we intend to silence the IKKα expression in prostate cancer cells using synthetic siRNAs and examine their biological effects on tumor cell invasiveness and growth.

Methods

Three synthetic siRNAs targeting different regions of the IKKα mRNA were designed, and the silencing effect was determined in PC-3 and DU145 cells. Numerous studies, including wound-healing assay, migration assay, invasion assay, cell attachment assay, cell proliferation, and cell cycle analysis, were conducted to investigate the biological effects of the IKKα siRNAs on prostate cancer cells.

Results

We have identified potent siRNAs that can silence the IKKα up to

SUBMITTER: Mahato R 

PROVIDER: S-EPMC4406877 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature