Simple Method for Markerless Gene Deletion in Multidrug-Resistant Acinetobacter baumannii.

ABSTRACT: The traditional markerless gene deletion technique based on overlap extension PCR has been used for generating gene deletions in multidrug-resistant Acinetobacter baumannii. However, the method is time-consuming because it requires restriction digestion of the PCR products in DNA cloning and the construction of new vectors containing a suitable antibiotic resistance cassette for the selection of A. baumannii merodiploids. Moreover, the availability of restriction sites and the selection of recombinant bacteria harboring the desired chimeric plasmid are limited, making the construction of a chimeric plasmid more difficult. We describe a rapid and easy cloning method for markerless gene deletion in A. baumannii, which has no limitation in the availability of restriction sites and allows for

SUBMITTER: Oh MH 

PROVIDER: S-EPMC4407223 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature