Humanization of the mouse mammary gland by replacement of the luminal layer with genetically engineered preneoplastic human cells.

ABSTRACT:

Introduction

The cell of origin for estrogen receptor α-positive (ERα+) breast cancer is probably a luminal stem cell in the terminal duct lobular units. To model these cells, we have used the murine myoepithelial layer in the mouse mammary ducts as a scaffold upon which to build a human luminal layer. To prevent squamous metaplasia, a common artifact in genetically-engineered breast cancer models, we sought to limit activation of the epidermal growth factor receptor (EGFR) during in vitro cell culture before grafting the cells.

Methods

Human reduction mammoplasty cells were grown in vitro in WIT medium. Epidermal growth factor in the medium was replaced with amphiregulin and neuregulin to decrease activation of EGFR and increase activation of EGFR homologs 3 and 4 (ERBB3 an

SUBMITTER: Verbeke S 

PROVIDER: S-EPMC4407301 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature