TETX: a novel nuclear selection marker for Chlamydomonas reinhardtii transformation.

ABSTRACT:

Background

Transformation of microalgae to obtain recombinant proteins, lipids or metabolites of economic value is of growing interest due to low costs associated with culture growth and scaling up. At present there are only three stable nuclear selection markers for the transformation of Chlamydomonas reinhardtii, which is the most commonly transformed microalgae, specifically: the aminoglycoside phosphotransferaseses aph7and aphVIII and the phleomycin resistance ble gene. As several microalgae are resistant to some of the antibiotics associated with the mentioned resistance genes, we have developed another alternative, tetX, a NADP-requiring Oxidoreductase that hydroxylates tetracycline substrates. We provide evidence that tetX can be used to obtain nuclear transformants of Chlam

SUBMITTER: Garcia-Echauri SA 

PROVIDER: S-EPMC4407551 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature