TREC-IN: gene knock-in genetic tool for genomes cloned in yeast.

ABSTRACT:

Background

With the development of several new technologies using synthetic biology, it is possible to engineer genetically intractable organisms including Mycoplasma mycoides subspecies capri (Mmc), by cloning the intact bacterial genome in yeast, using the host yeast's genetic tools to modify the cloned genome, and subsequently transplanting the modified genome into a recipient cell to obtain mutant cells encoded by the modified genome. The recently described tandem repeat coupled with endonuclease cleavage (TREC) method has been successfully used to generate seamless deletions and point mutations in the mycoplasma genome using the yeast DNA repair machinery. But, attempts to knock-in genes in some cases have encountered a high background of transformation due to maintenance of u

SUBMITTER: Chandran S 

PROVIDER: S-EPMC4407568 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature