High-throughput physical map anchoring via BAC-pool sequencing.

ABSTRACT:

Background

Physical maps created from large insert DNA libraries, typically cloned in BAC vector, are valuable resources for map-based cloning and de novo genome sequencing. The maps are most useful if contigs of overlapping DNA clones are anchored to chromosome(s), and ordered along them using molecular markers. Here we present a novel approach for anchoring physical maps, based on sequencing three-dimensional pools of BAC clones from minimum tilling path.

Results

We used physical map of wheat chromosome arm 3DS to validate the method with two different DNA sequence datasets. The first comprised 567 genes ordered along the chromosome arm based on syntenic relationship of wheat with the sequenced genomes of Brachypodium, rice and sorghum. The second dataset consisted of 7,13

SUBMITTER: Cvikova K 

PROVIDER: S-EPMC4407875 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature