Dual miRNA targeting restricts host range and attenuates neurovirulence of flaviviruses.

ABSTRACT: Mosquito-borne flaviviruses are among the most significant arboviral pathogens worldwide. Vaccinations and mosquito population control programs remain the most reliable means for flavivirus disease prevention, and live attenuated viruses remain one of the most attractive flavivirus vaccine platforms. Some live attenuated viruses are capable of infecting principle mosquito vectors, as demonstrated in the laboratory, which in combination with their intrinsic genetic instability could potentially lead to a vaccine virus reversion back to wild-type in nature, followed by introduction and dissemination of potentially dangerous viral strains into new geographic locations. To mitigate this risk we developed a microRNA-targeting approach that selectively restricts replication of flavivirus in the

SUBMITTER: Tsetsarkin KA 

PROVIDER: S-EPMC4408003 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature