Label free detection of 5'hydroxymethylcytosine within CpG islands using optical sensors.

ABSTRACT: Significant research has been invested in correlating genetic variations with different disease probabilities. Recently, it has become apparent that other DNA modifications, such as the addition of a methyl or hydroxymethyl group to cytosine, can also play a role. While these modifications do not change the sequence, they can negatively impact the function. Therefore, it is critical to be able to both read the genetic code and identify these modifications. Currently, the detection of hydroxymethylated cytosine (5'hmC) and the two closely related variants, cytosine (C) and 5'methylcytosine (5'mC), relies on a combination of nucleotide modification steps, followed by PCR and gene sequencing. However, this approach is not ideal because transcription errors which are inherent to the PCR proces

SUBMITTER: Hawk RM 

PROVIDER: S-EPMC4408228 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature