Progranulin protects against osteoarthritis through interacting with TNF-α and β-Catenin signalling.

ABSTRACT:

Objective

Progranulin (PGRN) was previously isolated as an osteoarthritis (OA)-associated growth factor. Additionally, PGRN was found to play a therapeutic role in inflammatory arthritis mice models through antagonising tumour necrosis factor α (TNF-α). This study was aimed at investigating the role of PGRN in degradation of cartilage and progression of OA.

Methods

Progression of OA was analysed in both spontaneous and surgically induced OA models in wild type and PGRN-deficient mice. Cartilage degradation and OA were evaluated using Safranin O staining, immunohistochemistry and ELISA. Additionally, mRNA expression of degenerative factors and catabolic markers known to be involved in cartilage degeneration in OA were analysed. Furthermore, the anabolic effects and underlying

SUBMITTER: Zhao YP 

PROVIDER: S-EPMC4408266 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature