ToF-SIMS imaging of lipids and lipid related compounds in Drosophila brain.

ABSTRACT: Drosophila melanogaster (fruit fly) has a relatively simple nervous system but possesses high order brain functions similar to humans. Therefore, it has been used as a common model system in biological studies, particularly drug addiction. Here, the spatial distribution of biomolecules in the brain of the fly was studied using time-of flight secondary ion mass spectrometry (ToF-SIMS). Fly brains were analyzed frozen to prevent molecular redistribution prior to analysis. Different molecules were found to distribute differently in the tissue, particularly the eye pigments, diacylglycerides, and phospholipids, and this is expected to be driven by their biological functions in the brain. Correlations in the localization of these molecules were also observed using principal components an

SUBMITTER: Phan NT 

PROVIDER: S-EPMC4408320 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature