Regulation of endogenous transmembrane receptors through optogenetic Cry2 clustering.

ABSTRACT: Transmembrane receptors are the predominant conduit through which cells sense and transduce extracellular information into intracellular biochemical signals. Current methods to control and study receptor function, however, suffer from poor resolution in space and time and often employ receptor overexpression, which can introduce experimental artefacts. We report a genetically encoded approach, termed Clustering Indirectly using Cryptochrome 2 (CLICR), for spatiotemporal control over endogenous transmembrane receptor activation, enabled through the optical regulation of target receptor clustering and downstream signalling using noncovalent interactions with engineered Arabidopsis Cryptochrome 2 (Cry2). CLICR offers a modular platform to enable photocontrol of the clustering of diverse trans

SUBMITTER: Bugaj LJ 

PROVIDER: S-EPMC4408875 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature