Establishment of a new quality control and vaccine safety test for influenza vaccines and adjuvants using gene expression profiling.

ABSTRACT: We have previously identified 17 biomarker genes which were upregulated by whole virion influenza vaccines, and reported that gene expression profiles of these biomarker genes had a good correlation with conventional animal safety tests checking body weight and leukocyte counts. In this study, we have shown that conventional animal tests showed varied and no dose-dependent results in serially diluted bulk materials of influenza HA vaccines. In contrast, dose dependency was clearly shown in the expression profiles of biomarker genes, demonstrating higher sensitivity of gene expression analysis than the current animal safety tests of influenza vaccines. The introduction of branched DNA based-concurrent expression analysis could simplify the complexity of multiple gene expression approach, an

SUBMITTER: Momose H 

PROVIDER: S-EPMC4409070 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature