Spectral properties of single gold nanoparticles in close proximity to biological fluorophores excited by 2-photon excitation.

ABSTRACT: Metallic nanoparticles (NPs) are able to modify the excitation and emission rates (plasmonic enhancement) of fluorescent molecules in their close proximity. In this work, we measured the emission spectra of 20 nm Gold Nanoparticles (AuNPs) fixed on a glass surface submerged in a solution of different fluorophores using a spectral camera and 2-photon excitation. While on the glass surface, we observed the presence in the emission at least 3 components: i) second harmonic signal (SHG), ii) a broad emission from AuNPS and iii) fluorescence arising from fluorophores nearby. When on the glass surface, we found that the 3 spectral components have different relative intensities when the incident direction of linear polarization was changed indicating different physical origins for these component

SUBMITTER: Anzalone A 

PROVIDER: S-EPMC4409109 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature