Mechanism of automaticity in cardiomyocytes derived from human induced pluripotent stem cells.

ABSTRACT:

Background and objectives

The creation of cardiomyocytes derived from human induced pluripotent stem cells (hiPS-CMs) has spawned broad excitement borne out of the prospects to diagnose and treat cardiovascular diseases based on personalized medicine. A common feature of hiPS-CMs is their spontaneous contractions but the mechanism(s) remain uncertain.

Methods

Intrinsic activity was investigated by the voltage-clamp technique, optical mapping of action potentials (APs) and intracellular Ca(2+) (Cai) transients (CaiT) at subcellular-resolution and pharmacological interventions.

Results

The frequency of spontaneous CaiT (sCaiT) in monolayers of hiPS-CMs was not altered by ivabradine, an inhibitor of the pacemaker current, If despite high levels of HCN transcripts (1-4).

SUBMITTER: Kim JJ 

PROVIDER: S-EPMC4409767 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature