In vitro and in vivo regulation of synaptogenesis by the novel antidepressant spadin.

ABSTRACT:

Background and purpose

We have described a novel antidepressant peptide, spadin, that acts by blocking the TWIK-related-potassium channel, type 1 (TREK-1). Here, we examined possible mechanisms of action of spadin at both molecular and cellular levels.

Experimental approaches

Effects of spadin were measured in primary cultures of neurons or tissues from mice injected i.v. with spadin. Western blots, qPCR, histochemical and electrophysiological techniques were used.

Key results

In vitro, spadin increased neuronal membrane potential and activated both the MAPK and PI3K signalling pathways, in a time- and concentration-dependent manner. The latter pathway was involved in the protective effect of spadin against staurosporine-induced apoptosis. Also, spadin enhanced both m

SUBMITTER: Devader C 

PROVIDER: S-EPMC4409910 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature