Validation of normal human bronchial epithelial cells as a model for influenza A infections in human distal trachea.

ABSTRACT: Primary normal human bronchial/tracheal epithelial (NHBE) cells, derived from the distal-most aspect of the trachea at the bifurcation, have been used for a number of studies in respiratory disease research. Differences between the source tissue and the differentiated primary cells may impact infection studies based on this model. Therefore, we examined how well-differentiated NHBE cells compared with their source tissue, the human distal trachea, as well as the ramifications of these differences on influenza A viral pathogenesis research using this model. We employed a histological analysis including morphological measurements, electron microscopy, multi-label immunofluorescence confocal microscopy, lectin histochemistry, and microarray expression analysis to compare differentiated NHBEs

SUBMITTER: Davis AS 

PROVIDER: S-EPMC4409941 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature