Cell-lineage heterogeneity and driver mutation recurrence in pre-invasive breast neoplasia.

ABSTRACT:

Background

All cells in an individual are related to one another by a bifurcating lineage tree, in which each node is an ancestral cell that divided into two, each branch connects two nodes, and the root is the zygote. When a somatic mutation occurs in an ancestral cell, all its descendants carry the mutation, which can then serve as a lineage marker for the phylogenetic reconstruction of tumor progression. Using this concept, we investigate cell lineage relationships and genetic heterogeneity of pre-invasive neoplasias compared to invasive carcinomas.

Methods

We deeply sequenced over a thousand phylogenetically informative somatic variants in 66 morphologically independent samples from six patients that represent a spectrum of normal, early neoplasia, carcinoma in situ, and

SUBMITTER: Weng Z 

PROVIDER: S-EPMC4410742 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature