Large-scale identification of core-fucosylated glycopeptide sites in pancreatic cancer serum using mass spectrometry.

ABSTRACT: Glycosylation has significant effects on protein function and cell metastasis, which are important in cancer progression. It is of great interest to identify site-specific glycosylation in search of potential cancer biomarkers. However, the abundance of glycopeptides is low compared to that of nonglycopeptides after trypsin digestion of serum samples, and the mass spectrometric signals of glycopeptides are often masked by coeluting nonglycopeptides due to low ionization efficiency. Selective enrichment of glycopeptides from complex serum samples is essential for mass spectrometry (MS)-based analysis. Herein, a strategy has been optimized using LCA enrichment to improve the identification of core-fucosylation (CF) sites in serum of pancreatic cancer patients. The optimized strategy was then

SUBMITTER: Tan Z 

PROVIDER: S-EPMC4410993 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature