Viral bimolecular fluorescence complementation: a novel tool to study intracellular vesicular trafficking pathways.

ABSTRACT: The Human Immunodeficiency Virus type 1 (HIV-1) accessory protein Nef interacts with a multitude of cellular proteins, manipulating the host membrane trafficking machinery to evade immune surveillance. Nef interactions have been analyzed using various in vitro assays, co-immunoprecipitation studies, and more recently mass spectrometry. However, these methods do not evaluate Nef interactions in the context of viral infection nor do they define the sub-cellular location of these interactions. In this report, we describe a novel bimolecular fluorescence complementation (BiFC) lentiviral expression tool, termed viral BiFC, to study Nef interactions with host cellular proteins in the context of viral infection. Using the F2A cleavage site from the foot and mouth disease virus we generated a vir

SUBMITTER: Dirk BS 

PROVIDER: S-EPMC4411132 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature