DNA extraction protocol for biological ingredient analysis of Liuwei Dihuang Wan.

ABSTRACT: Traditional Chinese medicine (TCM) preparations are widely used for healthcare and clinical practice. So far, the methods commonly used for quality evaluation of TCM preparations mainly focused on chemical ingredients. The biological ingredient analysis of TCM preparations is also important because TCM preparations usually contain both plant and animal ingredients, which often include some mis-identified herbal materials, adulterants or even some biological contaminants. For biological ingredient analysis, the efficiency of DNA extraction is an important factor which might affect the accuracy and reliability of identification. The component complexity in TCM preparations is high, and DNA might be destroyed or degraded in different degrees after a series of processing procedures. Therefore,

SUBMITTER: Cheng X 

PROVIDER: S-EPMC4411345 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature