Nucleotide variation and identification of novel blast resistance alleles of Pib by allele mining strategy.

ABSTRACT: Pib is one of significant rice blast resistant genes, which provides resistance to wide range of isolates of rice blast pathogen, Magnaporthe oryzae. Identification and isolation of novel and beneficial alleles help in crop enhancement. Allele mining is one of the best strategies for dissecting the allelic variations at candidate gene and identification of novel alleles. Hence, in the present study, Pib was analyzed by allele mining strategy, and coding and non-coding (upstream and intron) regions were examined to identify novel Pib alleles. Allelic sequences comparison revealed that nucleotide polymorphisms at coding regions affected the amino acid sequences, while the polymorphism at upstream (non-coding) region affected the motifs arrangements. Pib alleles from resistant landraces, Serc

SUBMITTER: Ramkumar G 

PROVIDER: S-EPMC4411389 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature