Use of Taguchi methodology to enhance the yield of caffeine removal with growing cultures of Pseudomonas pseudoalcaligenes.

ABSTRACT:

Background and objectives

Microbial caffeine removal is a green solution for treatment of caffeinated products and agro-industrial effluents. We directed this investigation to optimizing a bio-decaffeination process with growing cultures of Pseudomonas pseudoalcaligenes through Taguchi methodology which is a structured statistical approach that can be lowered variations in a process through Design of Experiments (DOE).

Material and methods

Five parameters, i.e. initial fructose, tryptone, Zn(+2) ion and caffeine concentrations and also incubation time selected and an L16 orthogonal array was applied to design experiments with four 4-level factors and one 3-level factor (4(4) × 1(3)). Data analysis was performed using the statistical analysis of variance (ANOVA) method. Furth

SUBMITTER: Ashengroph M 

PROVIDER: S-EPMC4411430 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature