The cistrome and gene signature of androgen receptor splice variants in castration resistant prostate cancer cells.

ABSTRACT:

Purpose

Spliced variant forms of androgen receptor were recently identified in castration resistant prostate cancer cell lines and clinical samples. We identified the cistrome and gene signature of androgen receptor splice variants in castration resistant prostate cancer cell lines and determined the clinical significance of androgen receptor splice variant regulated genes.

Materials and methods

The castration resistant prostate cancer cell line 22Rv1, which expresses full-length androgen receptor and androgen receptor splice variants endogenously, was used as the research model. We established 22Rv1-ARFL(-)/ARV(+) and 22Rv1-ARFL(-)/ARV(-) through RNA interference. Chromatin immunoprecipitation coupled with next generation sequencing and microarray techniques were used to id

SUBMITTER: Lu J 

PROVIDER: S-EPMC4411637 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature