Garlic (Allium sativum L.) fertility: transcriptome and proteome analyses provide insight into flower and pollen development.

ABSTRACT: Commercial cultivars of garlic, a popular condiment, are sterile, making genetic studies and breeding of this plant challenging. However, recent fertility restoration has enabled advanced physiological and genetic research and hybridization in this important crop. Morphophysiological studies, combined with transcriptome and proteome analyses and quantitative PCR validation, enabled the identification of genes and specific processes involved in gametogenesis in fertile and male-sterile garlic genotypes. Both genotypes exhibit normal meiosis at early stages of anther development, but in the male-sterile plants, tapetal hypertrophy after microspore release leads to pollen degeneration. Transcriptome analysis and global gene-expression profiling showed that >16,000 genes are differentially exp

SUBMITTER: Shemesh-Mayer E 

PROVIDER: S-EPMC4411974 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature