Intra-domain phage display (ID-PhD) of peptides and protein mini-domains censored from canonical pIII phage display.

ABSTRACT: In this paper, we describe multivalent display of peptide and protein sequences typically censored from traditional N-terminal display on protein pIII of filamentous bacteriophage M13. Using site-directed mutagenesis of commercially available M13KE phage cloning vector, we introduced sites that permit efficient cloning using restriction enzymes between domains N1 and N2 of the pIII protein. As infectivity of phage is directly linked to the integrity of the connection between N1 and N2 domains, intra-domain phage display (ID-PhD) allows for simple quality control of the display and the natural variations in the displayed sequences. Additionally, direct linkage to phage propagation allows efficient monitoring of sequence cleavage, providing a convenient system for selection and evolution of

SUBMITTER: Tjhung KF 

PROVIDER: S-EPMC4412080 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature