532 nm Low-Power Laser Irradiation Facilitates the Migration of GABAergic Neural Stem/Progenitor Cells in Mouse Neocortex.

ABSTRACT:

Background and objective

Accumulating evidence has shown that low-power laser irradiation (LLI) affects cell proliferation and survival, but little is known about LLI effects on neural stem/progenitor cells (NSPCs). Here we investigate whether transcranial 532 nm LLI affects NSPCs in adult murine neocortex and in neurospheres from embryonic mice.

Study design/materials and methods

We applied 532 nm LLI (Nd:YVO4, CW, 60 mW) on neocortical surface via cranium in adult mice and on cultured cells from embryonic mouse brains in vitro to investigate the proliferation and migration of NSPCs and Akt expression using immunohistochemical assays and Western blotting techniques.

Results

In vivo experiments demonstrated that 532 nm LLI significantly facilitated the migration of GA

SUBMITTER: Fukuzaki Y 

PROVIDER: S-EPMC4412395 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature