Mineralized collagen scaffolds induce hMSC osteogenesis and matrix remodeling.

ABSTRACT: Biomaterials for bone tissue engineering must be able to instruct cell behavior in the presence of the complex biophysical and biomolecular environments encountered in vivo. While soluble supplementation strategies have been identified to enhance osteogenesis, they are subject to significant diffusive loss in vivo or the need for frequent re-addition in vitro. This investigation therefore explored whether biophysical and biochemical properties of a mineralized collagen-GAG scaffold were sufficient to enhance human mesenchymal stem cell (hMSC) osteogenic differentiation and matrix remodeling in the absence of supplementation. We examined hMSC metabolic health, osteogenic and matrix gene expression profiles, as well as matrix remodeling and mineral formation as a function of scaffold mineral

SUBMITTER: Weisgerber DW 

PROVIDER: S-EPMC4412464 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature