Functional consequences for primary human alveolar macrophages following treatment with long, but not short, multiwalled carbon nanotubes.

ABSTRACT:

Purpose

Multiwalled carbon nanotubes (MWCNTs) are a potential human health hazard, primarily via inhalation. In the lung, alveolar macrophages (AMs) provide the first line of immune cellular defense against inhaled materials. We hypothesized that, 1 and 5 days after treating AMs with short (0.6 μm in length; MWCNT-0.6 μm) and long (20 μm in length; MWCNT-20 μm) MWCNTs for 24 hours, AMs would exhibit increased markers of adverse bioreactivity (cytokine release and reactive oxygen species generation) while also having a modified functional ability (phagocytosis and migration).

Methods

Primary human AMs were treated with short and long MWCNTs for 24 hours, 1 and 5 days after which toxicity end points, including cell death, reactive oxygen species generation, and inflammatory me

SUBMITTER: Sweeney S 

PROVIDER: S-EPMC4412488 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature